Systems Biology &
Bioengineering Research Laboratory
Leonidas G Alexopoulos, PhD
Dept. of Mechanical Engineering, National Technical University of Athens
Heroon Polytechniou 9,
15780 Zografou, Athens, GREECE
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Just came out: Identifying Drug Effects via Pathway Alterations using an Integer Linear Programming Optimization Formulation on Phosphoproteomic Data. Alexander Mitsos, Ioannis N. Melas, Paraskeuas Siminelakis, Aikaterini D. Chairakaki, Julio Saez-Rodriguez, Leonidas G. Alexopoulos (patent submitted). If you are interested screening your inhibitors and/or biologics using our technology please drop us a note. -------------------------------------------------------------------------------------------------------------------------- We are a multidisciplinary research laboratory composed of engineers, biologists and physicians working in the area of SYSTEMS BIOLOGY and BIOENGINEERING. We considered cells as dynamical systems and we apply novel biological and engineering appoaches in the area of drug discovery including phosphoproteomic-based drug target identification, reduction of generic pathways maps into data-driven pathways, and broad protein-based explorations of cell responses. BIOLOGICAL/PHARMACEUTICAL SIGNIFICANCE We apply novel experimental methods and engineering modeling in two main areas of biological significance: 1. CARTILAGE DEGENERATION (i.e. Osteoarthritis and Rheumatoid Arthritis) OPTIMAL TARGET IDENTIFICATION for Liver cancer based on phopshoproteomic data (i.e. identify targets for optimal clinical efficacy in Hepatocellular Carcinoma). EXPERIMENTAL APPROACHES 1. HIGH-THOUGHPUT PHOSPHOPROTEOMIC MEASUREMENTS: We can measure several intracelular and extracellular signaling events using novel experimental approaches coupled with mathematical modeling. For more information please refer to our review on the Drug Efficacy, Safety, and Biologics Discovery: Emerging Technologies and Tools (Wiley Series on Technologies for the Pharmaceutical Industry) by Sean Ekins and Jinghai J. Xu (Editors). 2. CUSTOM-MADE BEAD ASSAYS: We build an automation platform and manifacture our own coupling assays on magnetic beads for use with the Luminex xMAP platform. We are mostly interested on protein and phospoprotein targets via sandwich/ELISA bead-assays. 3. BIOMECHANICAL TESTING: We can quantify real-time the mechanical properties of cartilage and other soft tissues using biomechanical tests and custom-made FEM algorithms. Measuring mechanical properties of cartilage is a unique way to monitor its degeneration state. We also build a gadget to monitor real-time the mechanical properties of cartilage explants for real-time, in-vitro, high-throughout screening of drugs related to cartilage degeneration. FUNDING: We are grateful to the several sources of funding including Marie-Curie MIRG-14-CT-2007-046531, Max-Planck Univ. "Sugar Grant", Aachen Univ. DFG GSC111, and Vertex grant.
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